Effects of Gamisipjeon-tang on the Skin Regeneration of Deep Second Degree Burns in Mice= 심부 2도 화상을 유발한 쥐에서 가미십전탕이 피부 재생에 미치는 효과

학위논문(박사)-- 동국대학교 대학원: 한의학과, 2008. 8

Abstract
The Effects of Gamisipjeon-tang
on the Skin Regeneration of Deep Second Degree Burns in Mice
Hyun-Jung Yu
Dept. of Oriental Medicine
Graduate School of Dongguk University
(Directed by Prof. Seung-Ug Hong)
Objective : This study aimed to ascertain the curative effects of
Gamisipjeon-tang (GST) on the skin regeneration of deep second degree burns in
mice.
Material & Methods : In vitro, the IκB kinase (IKK) mRNA expression,
inducible nitric oxide synthase (iNOS) mRNA expression, and
cyclooxygenase-2 (COX-2) mRNA expression in the GST concentration from
1 ㎎ /㎖ to 10 ㎎ /㎖ were measured. In vivo, the mice were divided into four
groups : the normal group, the BE group (burn-elicited group, control group),
the DC group (Duoderm CGF-treated group after burn elicitation), and the
GST group (Gamisipjeon-tang treated group after burn elicitation). To
determine the anti-inflammatory effects, nuclear factor (NF)-κB p65, iNOS,
COX-2 positive reaction were measured by immunohistochemistry. To estimate
the skin regenerative effects, change of burn area, 5-bromo-2‘-deoxyuridine
(BrdU), and fibroblast growth factor (FGF) positive reaction were analyzed.
Results : In vitro, the iNOS, IKK, COX-2 mRNA expression decreased
according to the increase of GST concentration. The significant decrease of
- 2 -
COX-2, iNOS, NF-κB positive reaction were the highest in the GST group,
followed by the DC group and the BE group (p<0.05). The diameter of burn
area was significantly decreased in the GST group as compared to that in the
DC and BE group (p<0.05). The BrdU and FGF positive reaction increased
more significantly in the GST group than in the DC group, and more
significantly in the DC group than in the BE group on the 3rd and 7th day
after burn (p<0.05). FGF positive reaction increased in the BE and DC
group, whereas it decreased significantly in the GST group on the 14th day
(p<0.05). The BrdU positive reaction increased in the BE group, whereas it
decreased significantly in the DC and GST group on the 14th day (p<0.05).
Conclusions : This study shows that GST could decrease the inflammatory
response and accelerate the skin regeneration as compared to the duoderm
CGF in mice with deep second degree burns.
Key word : deep second degree burns, Gamisipjeon-tang (Jiaweishiquan-tang),
duoderm CGF, iNOS, NF-κB, FGF, BrdU.



이 논문은 유현정 박사가 특정 처방한약을 통해서 화상에 의한 피부손상에 대해서, 피부 재생 효과가 있음을 연구한 논문이다.